PERBEDAAN RERATA NILAI PCV ANTARA KELOMPOK PACKED RED CELL DARI TEKNIK PENGENDAPAN MANUAL DAN PACKED RED CELL DARI TEKNIK SENTRIFUGASI

Heru Setiawan; Intan Komalasari

doi:10.59946/jfki.2021.62

PERBEDAAN RERATA NILAI PCV ANTARA KELOMPOK PACKED RED CELL DARI TEKNIK PENGENDAPAN MANUAL DAN PACKED RED CELL DARI TEKNIK SENTRIFUGASI

Heru Setiawan
Intan Komalasari

DOI: https://doi.org/10.59946/jfki.2021.62

Abstrak

Komponen Packed Red Cell (PRC) merupakan terapi utama bagi penyakit keganasan hematologi, thalasemia, serta anemia aplastik. Komponen PRC diperoleh dengan menghilangkan sebagian besar plasma dari WB melalui proses sentrifugasi berpendingin. Jika tidak terdapat sentrifus berpendingin, dapat dilakukan melalui pengendapan manual di suhu selama 12 jam atau lebih. Komponen PRC yang telah dibuat harus memenuhi standar mutu yang telah ditentukan, dua diantaranya adalah konsentrasi Hb dan nilai PCV. Tujuan penelitian ini adalah untuk melihat perbedaaan bermakna nilai PCV dan Kadar Hb pada PRC dari teknik pengendapan manual selama 25 jam dengan teknik sentrifugasi pada kecepatan 4000 gforce selama 7 menit. Rancangan penelitian ini bersifat analitik komparatif, yaitu membandingkan hasil pemeriksaan PCV dan Hb pada PRC dari dua teknik pengendapan yang berbeda. Pemeriksaan PCV menggunakan metode mikroPCV dan Hb menggunakan metode fotometer dengan alat Hemocue 301⁺. Hasil uji didapatkan nilai PCV pada pengendapan manual berkisar 57,50% s/d 75% dengan nilai rata-rata 66,42% dan nilai SD 4,07, pada teknik sentrifugasi berkisar 56,50% s/d 74,0% dengan nilai rata-rata 65,72% dan nilai SD 4,01. Nilai Hb pada PRC pengendapan manual berkisar 17,55 g/dL s/d 25,30 g/dL dengan nilai rata-rata 21,39 dan nilai SD 1,89, pada teknik sentrifugasi berkisar 17,10 g/dL s/d 25,40 g/dL dengan nilai rata-rata 21,19 g/dL dan nilai SD 1,80. Uji statistik beda bermakna pada nilai PCV didapatkan nilai p 0,377 dan nilai p 0,913 pada konsentrasi Hb. Kesimpulan penelitian ini adalah tidak ada perbedaan bermakna nilai PCV dan Kadar Hb pada PRC dari teknik pengendapan manual 25 Jam dengan teknik sentrifugasi. Umumnya nilai PCV pada PRC sesuai standar, masing-masing sebanyak 70% pada pengendapan manual dan sentrifugasi. Begitu juga dengan konsentrasi Hb, sebanyak 100% PRC memenuhi standar pada pengendapan manual dan 96,7% memenuhi standar pada pengendapan sentrifugasi. Hal ini menunjukkan kedua metode pengendapan dapat digunakan

Referensi

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European Directorate for the Quality of Medicines. 2020. 20th Edition of the Guide to the preparation, use and quality assurance of blood components. European Committee on Blood Transfusion (CD-P-TS). Available at: PCVtps://www.edqm.eu/en/blood-guide.
Henschler, R. et al. 2010. Production of standard blood components. ISBT Science Series. p:190–195. doi: 10.1111/j.1751-2824.2010.01409.x.
Hussein, E. and Enein, A. 2014. Clinical and quality evaluation of red blood cell units collected via apheresis versus those obtained manually. Laboratory Medicine. pp. 238–243. doi: 10.1309/LMKXJ0Y44GPRSXFG.
Ilhami, T. et al. 2014. Kualitas dan Potensi Hemolisis Packed Red Cell (PRC) Washed Erythrocyte dan Leukodepleted ( In-Line ) dalam Transfusi Klinis. J Indon Med Assoc. pp. 451–455.
McCullough, J. 2011. Transfusion Medicine: Third Edition, Transfusion Medicine: Third Edition. doi: 10.1002/9781444398748.
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NACO New Delhi. 2015. Buku Pedoman Persiapan Komponen untuk BCSU.
Nency, Y. M. and Sumanti, D. 2016. Latar Belakang Penyakit pada Penggunaan Transfusi Komponen Darah pada Anak. Sari Pediatri, 13(3), p. 159. doi: 10.14238/sp13.3.2011.159-64
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Sulung, N., Fuadda, R. and Juwita, L. V. 2018. Perbedaan Reaksi Pemberian Transfusi Darah Whoole Blood (Wb) Dan Packed Red Cell (PRC) Pada Pasien Sectio Caesare. Human Care Journal. 1(3). doi : 10.32883/hcj.v1i3.29.

Jurnal Fisioterapi dan Kesehatan Indonesia

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Setiawan, H., & Komalasari, I. (2021). PERBEDAAN RERATA NILAI PCV ANTARA KELOMPOK PACKED RED CELL DARI TEKNIK PENGENDAPAN MANUAL DAN PACKED RED CELL DARI TEKNIK SENTRIFUGASI. Jurnal Fisioterapi Dan Kesehatan Indonesia, 1(2), 132-136. https://doi.org/10.59946/jfki.2021.62

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All copies, print or electronic, or other use of the paper or article must include the appropriate bibliographic citation for the article’s publication in the journal.

2. Requests from third parties

Although authors are permitted to re-use all or portions of the article in other works, this does not include granting third-party requests for reprinting, republishing, or other types of re-use. Requests for all uses not included above, including the authorization of third parties to reproduce or otherwise use all or part of the article (including figures and tables), should be referred to RCEPM-LIPI by going to our website athttp://telimek.lipi.go.id.

3. Author Online Use

Personal Servers. Authors and/or their employers shall have the right to post the accepted version of articles pre-print version of the article, or revised personal version of the final text of the article (to reflect changes made in the peer review and editing process) on their own personal servers or the servers of their institutions or employers without permission from RCEPM-LIPI, provided that the posted version includes a prominently displayed RCEPM-LIPI copyright notice and, when published, a full citation to the original publication, including a link to the article abstract in the journal homepage. Authors shall not post the final, published versions of their papers;
Classroom or Internal Training Use. An author is expressly permitted to post any portion of the accepted version of his/her own articles on the author’s personal web site or the servers of the author’s institution or company in connection with the author’s teaching, training, or work responsibilities, provided that the appropriate copyright, credit, and reuse notices appear prominently with the posted material. Examples of permitted uses are lecture materials, course packs, e-reserves, conference presentations, or in-house training courses;
Electronic Preprints. Before submitting an article to an MEV Journal, authors frequently post their manuscripts to their own web site, their employer’s site, or to another server that invites constructive comment from colleagues. Upon submission of an article to MEV Journal, an author is required to transfer copyright in the article to RCEPM-LIPI, and the author must update any previously posted version of the article with a prominently displayed RCEPM-LIPI copyright notice. Upon publication of an article by the RCEPM-LIPI, the author must replace any previously posted electronic versions of the article with either (1) the full citation to the work with a Digital Object Identifier (DOI) or link to the article abstract in MEV journal homepage, or (2) the accepted version only (not the final, published version), including the RCEPM-LIPI copyright notice and full citation, with a link to the final, published article in journal homepage.

4. Articles in Press (AiP) service

RCEPM-LIPI may choose to publish an abstract or portions of the paper before we publish it in the journal. Please contact our Production department immediately if you do not want us to make any such prior publication for any reason, including disclosure of a patentable invention.

5. Author/Employer Rights

If you are employed and prepared the article on a subject within the scope of your employment, the copyright in the article belongs to your employer as a work-for-hire. In that case, RCEPM-LIPI assumes that when you sign this Form, you are authorized to do so by your employer and that your employer has consented to the transfer of copyright, to the representation and warranty of publication rights, and to all other terms and conditions of this Form. If such authorization and consent has not been given to you, an authorized representative of your employer should sign this Form as the Author.

6. RCEPM-LIPI Copyright Ownership

It is the formal policy of RCEPM-LIPI to own the copyrights to all copyrightable material in its technical publications and to the individual contributions contained therein, in order to protect the interests of the RCEPM-LIPI, its authors and their employers, and, at the same time, to facilitate the appropriate re-use of this material by others. RCEPM-LIPI distributes its technical publications throughout the world and does so by various means such as hard copy, microfiche, microfilm, and electronic media. It also abstracts and may translate its publications, and articles contained therein, for inclusion in various compendiums, collective works, databases and similar publications

Every accepted manuscript should be accompanied by "Copyright Transfer Agreement" prior to the article publication.