Differences in the Bisection Line in the Improved Neubauer Counting Booth on the Appropriateness of Leukocyte Count
Abstract
Leukocyte count examination is an examination that is often performed in clinical laboratories. Leukocyte count examination can be performed using a hematology analyzer or counting chamber for manual methods. Leukocyte cell counting using the manual method must use a standardized counting chamber. Currently, a different type of Improved Neubauer counting chamber variation has been found from the standard type, namely the difference in the outer limit of the counting area. In the standard type counting chamber, the outer limit of the counting area has three lines, while in the variation type counting chamber there is only one line. The purpose of this study was to determine whether there was a difference in the dividing line of the Improved Neubauer counting chamber on the suitability of the leukocyte count between the standard Improved Neubauer counting chamber type and the variation type Improved Neubauer counting chamber. The research design used in this study was observational analytic (Cross-Sectional) with a sample size of 20 EDTA blood. The results of the study obtained normally distributed data. then the Dependent Parametric Test (Paired T-Test) was carried out with a confidence level of 95%. The results of the Paired T-test showed the Sig.(2-tailed) value was 0.000 (<0.05). The conclusion of the study was that there was a significant difference in the results of the leukocyte count between the use of the standard Improved Neubauer counting chamber and the variation Improved Neubauer counting chamber. The use of the standard Improved Neubauer counting chamber is more recommended than the variation Improved Neubauer counting chamber.
References
Risky novia rahayu. Gambaran Hitung Julah Leukosit Dan Immature/Total Neutrophil Ratio (I/T Ratio) Pada Neonatus Diduga Sepsis Di RSUD Dr Chasbullah Abdulmadjid Kota; 2019
Amaniyah Muti K. Perbedaan Penggunaan Antikoagulan NaEDTA, K2EDTA Dan K3EDTA Terhadap Profil Leukosit Yang Diperiksa Dengan Hematology Analyzer. 2021
Ariyadi T, Sukeksi A. Perbedaan Jumlah Lekosit Sampel Segera Diperiksa Dan Tunda 2 Jam Dan 4 jam Pada Pasien Lekositosis [Internet]. Available from: http://repository.unimus.ac.id. 2018.
Nur M, Harjoko A, Candradewi I. Klasifikasi Sel Darah Putih Berdasarkan Ciri Warna dan Bentuk dengan Metode K-Nearest Neighbor (K-NN). Vol.6, No.2, pp. 151~162; 2016.
Labmart. Counting Chamber for Hemacytometer, Germany (not medical device, only for professional lab use) [Internet]. Labmart. 2024 [cited 2024 May 1]. Available from: https://mylabmart.com/shop/counting-chamber-for-hemacytometer
Asiyah, Nur. Perbedaan Jumlah Leukosit Sampel Segera Diperiksa Dan Tunda 2 Jam Dan 4 Jam Pada Pasien Leukositosis. Manuscript.2018
dr.Fadli R. Tes Hematologi Rutin [Internet]. halodoc. 2022. [cited 2024 May 1]. Available from: https://www.halodoc.com/kesehatan/tes-hematologi-rutin
Iverson BL, Dervan PB. Hemostasis. 2018;7823–30.
Ayu Eva Maharani, Astuti Dewi. Buku Penuntun Praktikum Hematologi I Bagi Mahasiswa DIV. Modul.2016
Sugiyono. METLIT SUGIYONO.pdf. 2015. p. 3361.
Astuti Dewi, Ayu Eva Maharani. Buku Penuntun Praktikum Hematologi III Bagi Mahasiswa DIV. Modul.2018
Ahmad, Hanifa. Hubungan Hitung Neutrofil Dan Limfosit Terhadap Keparahan Penyakit Pada Pasien Covid-19 Di Rumah Sakit X Di Jawa Barat. Skirpsi. 2021
Raflanda, Hamidah. Perbandingan Hasil Hitung Jenis Leukosit Metode Manual Dengan Metode Otomatisasi Flowsitometri. Skirpsi. 2019
Notoatmodjo S. Metodologi penelitian kesehatan (2018) [Internet]. perpustakaan terpadu polkestama.2018p.243. [cited 2024 May 1]. Available from:http://opac.poltekkestasikmalaya.ac.id/index.php?p=show_detail&id=3190
Mushlih M, Rosyadi R. Buku Ajar Mata Kuliah Statistika “Aplikasi di Dunia Kesehatan”. Universitas Muhammmadiyah Sidoarjo 2020. Sidoarjo. UMSIDA Press; 2020
Sopiyudin M Dahlan. Besar Sampel Dan Cara Pengambilan Sampel Dalam Penelitian Kedokteran dan Kesehatan. Edisi 3 seri evidence based medicine 2. Jakarta. 2018
Zhang M, Lingui Gu, Zheng Peihua, Chen Zhixin, Dou Xinqi, qin Qizhong. Improvement Of Cell Counting Method For Neubauer Counting Chamber. [Internet]. Journal of Clinical laboratory Analysis/Volume 34, Issue 1/e23024 [cited 2024 june 26]. Available from: https://onlinelibrary.wiley.com/doi/10.1002/jcla.23024
Copyright (c) 2024 IFI Cabang Kota Bekasi
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Submission Preparation Checklist
As part of the submission process, authors are required to check off their submission's compliance with all of the following items, and submissions may be returned to authors that do not adhere to these guidelines.
- I hereby declare that this submission is my own work.
- I hereby stated that this manusrcipt have no plagiarism matter.
- I declare the submission has no potential conflict of interest.
- I hereby stated that this manusrcipt have never been previously published in any other scientific publication and not being under reviewing process of any other scientific publication.
- The submitted manuscript contains no least than 2.000 words and not exceed 25 pages A4 including figures and tables, without any appendixes.
- The submitted manuscript has been written using Open Office Text Document (.odt) or Microsoft Word (.doc/.docx).
- Title already brief and concise, written in English, and less than 15 words.
- Abstract already brief and concise and not exceed 150 words in English.
- Keywords are written in English, between three to five phrase.
- The manuscript structure already consist: Introduction, Method/Material, Result and Discussion, Conclusion, Acknowledgement, and References.
- References are written according the writing style of MEV. The primary references are no less than 80% from at least fifteen sources and had been taken from the late ten year publications.
- The instructions in Ensuring a Blind Review have been followed to the submitted manuscript.
- (At least) two scientific referees have been suggested in "Comments for the Editor" textbox in the bottom of this page.
Copyright Notice
Retained Rights/Terms and Conditions of Publication
1. As an author you (or your employer or institution) may do the following:
- make copies (print or electronic) of the article for your own personal use, including for your own classroom teaching use;
- make copies and distribute such copies (including through e-mail) of the article to research colleagues, for the personal use by such colleagues (but not commercially or systematically, e.g. via an e-mail list or list server);
- present the article at a meeting or conference and to distribute copies of the article to the delegates attending such meeting;
- for your employer, if the article is a ‘work for hire’, made within the scope of your employment, your employer may use all or part of the information in the article for other intra-company use (e.g. training);
- retain patent and trademark rights and rights to any process, procedure, or article of manufacture described in the article;
- include the article in full or in part in a thesis or dissertation (provided that this is not to be published commercially);
- use the article or any part thereof in a printed compilation of your works, such as collected writings or lecture notes (subsequent to publication of the article in the journal); and prepare other derivative works, to extend the article into book-length form, or to otherwise re-use portions or excerpts in other works, with full acknowledgement of its original publication in the journal;
- may reproduce or authorize others to reproduce the article, material extracted from the article, or derivative works for the author’s personal use or for company use, provided that the source and the copyright notice are indicated, the copies are not used in any way that implies RCEPM-LIPI endorsement of a product or service of any employer, and the copies themselves are not offered for sale.
All copies, print or electronic, or other use of the paper or article must include the appropriate bibliographic citation for the article’s publication in the journal.
2. Requests from third parties
Although authors are permitted to re-use all or portions of the article in other works, this does not include granting third-party requests for reprinting, republishing, or other types of re-use. Requests for all uses not included above, including the authorization of third parties to reproduce or otherwise use all or part of the article (including figures and tables), should be referred to RCEPM-LIPI by going to our website athttp://telimek.lipi.go.id.
3. Author Online Use
- Personal Servers. Authors and/or their employers shall have the right to post the accepted version of articles pre-print version of the article, or revised personal version of the final text of the article (to reflect changes made in the peer review and editing process) on their own personal servers or the servers of their institutions or employers without permission from RCEPM-LIPI, provided that the posted version includes a prominently displayed RCEPM-LIPI copyright notice and, when published, a full citation to the original publication, including a link to the article abstract in the journal homepage. Authors shall not post the final, published versions of their papers;
- Classroom or Internal Training Use. An author is expressly permitted to post any portion of the accepted version of his/her own articles on the author’s personal web site or the servers of the author’s institution or company in connection with the author’s teaching, training, or work responsibilities, provided that the appropriate copyright, credit, and reuse notices appear prominently with the posted material. Examples of permitted uses are lecture materials, course packs, e-reserves, conference presentations, or in-house training courses;
- Electronic Preprints. Before submitting an article to an MEV Journal, authors frequently post their manuscripts to their own web site, their employer’s site, or to another server that invites constructive comment from colleagues. Upon submission of an article to MEV Journal, an author is required to transfer copyright in the article to RCEPM-LIPI, and the author must update any previously posted version of the article with a prominently displayed RCEPM-LIPI copyright notice. Upon publication of an article by the RCEPM-LIPI, the author must replace any previously posted electronic versions of the article with either (1) the full citation to the work with a Digital Object Identifier (DOI) or link to the article abstract in MEV journal homepage, or (2) the accepted version only (not the final, published version), including the RCEPM-LIPI copyright notice and full citation, with a link to the final, published article in journal homepage.
4. Articles in Press (AiP) service
RCEPM-LIPI may choose to publish an abstract or portions of the paper before we publish it in the journal. Please contact our Production department immediately if you do not want us to make any such prior publication for any reason, including disclosure of a patentable invention.
5. Author/Employer Rights
If you are employed and prepared the article on a subject within the scope of your employment, the copyright in the article belongs to your employer as a work-for-hire. In that case, RCEPM-LIPI assumes that when you sign this Form, you are authorized to do so by your employer and that your employer has consented to the transfer of copyright, to the representation and warranty of publication rights, and to all other terms and conditions of this Form. If such authorization and consent has not been given to you, an authorized representative of your employer should sign this Form as the Author.
6. RCEPM-LIPI Copyright Ownership
It is the formal policy of RCEPM-LIPI to own the copyrights to all copyrightable material in its technical publications and to the individual contributions contained therein, in order to protect the interests of the RCEPM-LIPI, its authors and their employers, and, at the same time, to facilitate the appropriate re-use of this material by others. RCEPM-LIPI distributes its technical publications throughout the world and does so by various means such as hard copy, microfiche, microfilm, and electronic media. It also abstracts and may translate its publications, and articles contained therein, for inclusion in various compendiums, collective works, databases and similar publications
Every accepted manuscript should be accompanied by "Copyright Transfer Agreement" prior to the article publication.